Gene description for ACTR3
Gene name actin related protein 3
Gene symbol ACTR3
Other names/aliases -
Species Bos taurus
 Experiment description of studies that identified ACTR3 in Bovine milk

1
Experiment ID439
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22129587   
OrganismBos taurus
Experiment descriptionBovine milk exosome proteome.
AuthorsReinhardt TA, Lippolis JD, Nonnecke BJ, Sacco RE.
Journal name J Proteomics
Publication year2012
SampleBovine milk
Sample nameNormal cow-Milk
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120041: EV-METRIC:56%

2
Experiment ID503
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23459212   
OrganismBos taurus
Experiment descriptionBovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis.
AuthorsReinhardt TA, Sacco RE, Nonnecke BJ, Lippolis JD.
Journal name J Proteomics
Publication year2013
SampleBovine milk
Sample nameStaphylococcus aureus-infected-Milk
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130111: EV-METRIC:43%

3
Experiment ID1045
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28725021   
OrganismBos taurus
Experiment descriptionBovine milk-derived exosomes from colostrum are enriched with proteins implicated in immune response and growth.
AuthorsSamuel M, Chisanga D, Liem M, Keerthikumar S, Anand S, Ang CS, Adda CG, Versteegen E, Jois M, Mathivanan S.
Journal name Sci Rep.
Publication year2017
SampleBovine milk
Sample nameBovine milk - colostrum 12 h and 24 h
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.08.1.24 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

4
Experiment ID1046
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28725021   
OrganismBos taurus
Experiment descriptionBovine milk-derived exosomes from colostrum are enriched with proteins implicated in immune response and growth.
AuthorsSamuel M, Chisanga D, Liem M, Keerthikumar S, Anand S, Ang CS, Adda CG, Versteegen E, Jois M, Mathivanan S.
Journal name Sci Rep.
Publication year2017
SampleBovine milk
Sample nameBovine milk - colostrum 72 h
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.08.1.24 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

5
Experiment ID1047
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28725021   
OrganismBos taurus
Experiment descriptionBovine milk-derived exosomes from colostrum are enriched with proteins implicated in immune response and growth.
AuthorsSamuel M, Chisanga D, Liem M, Keerthikumar S, Anand S, Ang CS, Adda CG, Versteegen E, Jois M, Mathivanan S.
Journal name Sci Rep.
Publication year2017
SampleBovine milk
Sample nameBovine milk - colostrum 48 h
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.08.1.24 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

6
Experiment ID1048
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28725021   
OrganismBos taurus
Experiment descriptionBovine milk-derived exosomes from colostrum are enriched with proteins implicated in immune response and growth.
AuthorsSamuel M, Chisanga D, Liem M, Keerthikumar S, Anand S, Ang CS, Adda CG, Versteegen E, Jois M, Mathivanan S.
Journal name Sci Rep.
Publication year2017
SampleBovine milk
Sample nameBovine milk - mature milk
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.08.1.24 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

7
Experiment ID2815
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34168137   
OrganismBos taurus
Experiment descriptionOral administration of bovine milk-derived extracellular vesicles induces senescence in the primary tumor but accelerates cancer metastasis
AuthorsSamuel M, Fonseka P, Sanwlani R, Gangoda L, Chee SH, Keerthikumar S, Spurling A, Chitti SV, Zanker D, Ang CS, Atukorala I, Kang T, Shahi S, Marzan AL, Nedeva C, Vennin C, Lucas MC, Cheng L, Herrmann D, Pathan M, Chisanga D, Warren SC, Zhao K, Abraham N, Anand S, Boukouris S, Adda CG, Jiang L, Shekhar TM, Baschuk N, Hawkins CJ, Johnston AJ, Orian JM, Hoogenraad NJ, Poon IK, Hill AF, Jois M, Timpson P, Parker BS, Mathivanan S.
Journal name Nat Commun
Publication year2021
SampleBovine milk
Sample nameRaw milk
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
mRNA
miRNA
tRNA
Methods used in the studyWestern blotting
Mass spectrometry
Small RNA sequencing
EV-TRACK -

8
Experiment ID2822
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34168137   
OrganismBos taurus
Experiment descriptionOral administration of bovine milk-derived extracellular vesicles induces senescence in the primary tumor but accelerates cancer metastasis
AuthorsSamuel M, Fonseka P, Sanwlani R, Gangoda L, Chee SH, Keerthikumar S, Spurling A, Chitti SV, Zanker D, Ang CS, Atukorala I, Kang T, Shahi S, Marzan AL, Nedeva C, Vennin C, Lucas MC, Cheng L, Herrmann D, Pathan M, Chisanga D, Warren SC, Zhao K, Abraham N, Anand S, Boukouris S, Adda CG, Jiang L, Shekhar TM, Baschuk N, Hawkins CJ, Johnston AJ, Orian JM, Hoogenraad NJ, Poon IK, Hill AF, Jois M, Timpson P, Parker BS, Mathivanan S.
Journal name Nat Commun
Publication year2021
SampleBovine milk
Sample nameCommercial milk
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
mRNA
miRNA
tRNA
Methods used in the studyMass spectrometry
Small RNA sequencing
EV-TRACK -

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